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. Author manuscript; available in PMC: 2022 Oct 7.
Published in final edited form as: Genesis. 2021 Oct 7;59(10):e23452. doi: 10.1002/dvg.23452

Figure 2.

Figure 2.

Identification of furrow-localized germ plasm marker nanos3 in multiple Danionin species. Diagram (A) and micrographs (B-E) visualizing top view (blastodisc) of 4-cell Danionin fish embryos with nanos3 mRNA labeled via fluorescence in situ hybridization. The general appearance and localization pattern of the germ plasm marker nanos3 at the distal end of the first two cleavage furrows is consistent across all four species, including Danio rerio (B), Danio kyathit (C), Danio albolineatus (D), and Devario aequipinnatus (E). A nanos3 germ plasm aggregate in each embryo is highlighted by a green box outline. Micrographs are 2D maximum projections of confocal Z-stacks.