Figure 5.

RNA localization at the distal ends of cleavage furrows in four cell stage Danio rerio, D. kyathit, D. albolineatus, and Devario aequipinnatus embryos as visualized by fluorescence in situ hybridization and confocal microscopy. All 12 known RNA components of zebrafish germ plasm are also germ plasm localized in Danio kyathit and Danio albolineatus, while all except ca15b are germ plasm localized in Devario aequipinnatus (yellow box; see also Figure 6). Furrow localization and/or labeling of dazl RNA in Devario aequipinnatus during the early cleavage stages was weak and difficult to visualize at 10x magnification (indicated by white asterisk) but could be observed at higher magnification (Sup. Fig. 3). All micrographs are 2D maximum projections of confocal stacks.