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. Author manuscript; available in PMC: 2022 Oct 7.
Published in final edited form as: Genesis. 2021 Oct 7;59(10):e23452. doi: 10.1002/dvg.23452

Table 2.

Primers used for in situ probe synthesis with T7 RNAP promoter underlined

Probe target Forward primer Reverse primer Source of
template
ca15b_exon8-9 TGCAATGAAGGTGTGATCTGG GAAATTAATACGACTCACTATAGGATTGACTGGCTGAACGGATC D. rerio cDNA (4 cell)
ca15b_exon8-9 *sense probe GAAATTAATACGACTCACTATAGG TGCAATGAAGGTGTGATCTGG TTGACTGGCTGAACGGATC D. rerio cDNA (4 cell)
ca15b_exon4 (Devario) CATGGCCCATCATTGCAGAA GAAATTAATACGACTCACTATAGGGCCAACGTATGTGAAAGAGGT Devario DNA (adult fin clip)
ca15b_exon8-9 (Devario) TGCAATGAAGGTGTGATCTGG GAAATTAATACGACTCACTATAGGATTGACTGGCTGAACGGATC Devario cDNA (2 cell)
celf1 GACCAGCCCGACATTGATTC GAAATTAATACGACTCACTATAGGTCTGTGACTGGTGCATGGAT D. rerio cDNA (4 cell)
granulito GACCAATAGAAAAACCCGACAAC GAAATTAATACGACTCACTATAGGTGCCATCTTAACTAGGAAATCCC D. rerio cDNA (4 cell)
hook2 CGCAGACAAAACACACTCGA GAAATTAATACGACTCACTATAGGTCTTTCTCCGACAGCTGGTT D. rerio cDNA (4 cell)
rgs14a ATCAGACAGCAAGACCAGC GAAATTAATACGACTCACTATAGGGATCCAGACACCAACTCCAG D. rerio cDNA (4 cell)
tdrd7a AATCACCAGTGCCTGAGAAG GAAATTAATACGACTCACTATAGGATTCGACCCTGAACCAACTC D. rerio cDNA (4 cell)