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. 2021 Dec 3;14:6523–6542. doi: 10.2147/JIR.S339670

Figure 2.

Figure 2

Vitamin D does not exert joint protective effect in DMM-induced OA VDR−/− mice model. (A) Severity of DMM-induced OA VDR−/− mice model as determined by Safranin-O and Fast Green, and micro-CT assays. Expression levels of COL2A1, aggrecan, MMP13 and ADAMTS5 as determined by immunofluorescence staining. Scale bar = 400 μm for Safranin-O and Fast Green staining; Scale bar = 100 μm for immunofluorescence staining. (B) OARIS scores in each group (n = 10, one-way ANOVA). (C) Osteophyte score in each group (n = 10, one-way ANOVA). (D) Quantification of mean fluorescence intensity of COL2A1, aggrecan, MMP13 and ADAMTS5 in each group (n = 10, one-way ANOVA). (E) ELISA analysis of IL-1β, IL-18 and TNF-α levels in mice knee synovial fluid (n = 3, one-way ANOVA). Data are presented as mean ± SD. ns, no significance; **p <0.01.