Figure 4: Confocal Microscopy Images of Stable and Ruptured Human Coronary Arteries from Carriers and Non-Carriers of APOL1 Risk Alleles.
Representative confocal microscopy images with staining against APOL1 (red) CD68 (green), and DAPI (blue) in stable (A) and in ruptured (B) coronary plaques. APOL1-positive areas were larger in carriers of two APOL1 risk alleles than in non-carriers of APOL1 risk alleles in stable coronary artery plaques (including 19 carriers of the reference allele, 15 carriers of one APOL1 risk allele and 7 carriers of two APOL1 risk allele) (C) and in ruptured plaques (including 13 carriers of the reference allele, 7 carriers of one APOL1 risk allele and 6 carriers of two APOL1 risk alleles) (E). There was no difference in macrophage inflammation, as assessed by staining against CD68 in stable (D) and in ruptured coronary plaques (F).