FIG 3.

TraAB OE suppresses cell reversals independently of the Frz chemosensory pathway. (A) Digitally labeled trajectories of marked cells from Movie S2 in the supplemental material. Each colored curve represents a trajectory of one cell; 443 cells were tracked for the duration of the whole movie (60 min at 1-min intervals). (B) Run duration distribution of the tracked cells. The vast majority of cells did not reverse during the observation. (C) CA formation occurs independently of FrzCD methylation changes. Negative control (ΔfrzCD), parent, and TraAB OE strains were harvested from indicated agar plates grown for 24 h. Only the TraAB OE strain with 2 mM IPTG formed CAs. Representative immunoblot probed with α-FrzCD serum shown. Left, molecular weight standards. *, nonspecific loading control band.