TABLE 1.
Plasmids, strains, and primers used in this study
| Plasmid, strain, or primer name | Relevant features or sequencea (5′→3′) | Figure(s) and/or movie(s) | Source or reference |
|---|---|---|---|
| Plasmids | |||
| pCV10 | pSWU19-PR3+4-eGFP, Kmr | 46 | |
| pDP21 | PpilA-traAB in pSWU19 (Mx8 attP), Kmr | 18 | |
| pDP22 | PpilA in pSWU19, Kmr | 61 | |
| pMR3487 | IPTG-inducible promoter, Tcr | 55 | |
| ptdTomato | pMR3487-tdTomato, Tcr | Larry Shimkets | |
| pPC4 | PpilA-traABMf in pSWU19, Kmr | 16 | |
| pPC57 | PpilA-(ATG)-traAB in pSWU19, Kmr | This study | |
| pPC58 | pMR3487-(ATG)-traAB, Tcr | This study | |
| pPC59 | PpilA-(ATG)-traAB(ΔOmpA) in pSWU19, Kmr | This study | |
| Strains | |||
| TOP10 | E. coli cloning strain | Invitrogen | |
| DK1622 | Wild-type M. xanthus; all strains derived | 62 | |
| DK8601 | DK1622 aglB1 (aglQ1) ΔpilA::tc, nonmotile, Tcr | Fig. 1 (top left) | 62, 63 |
| DW1463 | DK8601 (pDP21), Kmr, Tcr | Fig. 1 (bottom left) | 18, 63 |
| DK10410 | DK1622 ΔpilA (markerless) | Fig. 1 (top middle and right), Fig. 3C, Fig. S1A (top), Fig. S4, Fig. S5, Fig. S7B, Movie S1 | 18, 64 |
| DW2292 | DK10410 (pPC57), Kmr | Fig. 1 (bottom middle and right), Fig. 3A and B, Fig. S1A (bottom), Fig. S2 to S6, Movies S1 and S2 | This study |
| DW2293 | DW2292 (ptdTomato), Kmr, Tcr | Fig. 3A and B, Fig. S2, Fig. S6A and B, Movie S2 | This study |
| DW2294 | DK10410 (pPC58), Tcr | Fig. 3C, Fig. S1B | This study |
| DW712 | DK1622 ΔfrzA-E::km ΔpilA::tc, Kmr, Tcr | Fig. S4B | This study |
| DW1466 | DK1622 ΔcglC Δtgl (markerless) | Fig. S3B | 18 |
| DW1483 | DK8601 ΔtraAB (markerless) | Fig. S3B | 16, 18 |
| DW2296 | DW1463 (pPC57), Kmr, Tcr | Fig. S3B | This study |
| DW2297 | DW1483 (pPC59), Kmr, Tcr | Fig. S3B | This study |
| DW2298 | DW2270 (pPC59), Kmr | Fig. S3A and C | This study |
| DW2270 | DK10410 ΔtraAB (markerless) | Fig. S3A and C | 17 |
| DW2295 | DK10410 (ptdTomato), Tcr | Fig. 5E, Fig. S7 | This study |
| DW2539 | DW2294 ΔfrzA-E::km, Kmr, Tcr | Fig. 3C | This study |
| DW2538 | DW2295 ΔfrzA-E::km, Kmr, Tcr | Fig. 5F, Fig. S7 | This study |
| DW2300 | DW2270 (pPC4, ptdTomato), Kmr, Tcr | Fig. 5D, Fig. S7 | This study |
| DW2540 | DW2294 (pCV10), Kmr, Tcr | Fig. 5D to F, Fig. S7 | This study |
| Primers | |||
| (ATG)-TraA-RBS-XbaI-F | GACGACTCTAGAGGAAACCAAGAATAGAAATAGAAAGGAGAATTAATGGGAGATATCCCTCATTG | ||
| TraB-HindIII-R | GACGACAAGCTTGGAGTTCTTCACCTCGGACTC | ||
| XbaI-(ATG)-TraA-F | GGATAACAATTAAGGAGGCTCTAGAATGGGAGATATCCCTCATTG | ||
| TraB-KpnI-R | TGATTACGAAGGCGAGCTCGGTACCGGAGTTCTTCACCTCGGACT | ||
| pSWU19-EcoRI-PpilA-F | AGGAAACAGCTATGACCATGATTACGAATTCCGTCATGTTGGACGAGGT | ||
| TraB-ΔOmpA-R | GAAGTCGACGCGGTTGAGCTGCTCCAGGATGACAAT | ||
| TraB-ΔOmpA-F | GGAGCAGCTCAACCGCGTCGACTTCACCATCC | ||
| pSWU19-XbaI-HindIII-TraB-R | TGCATGCCTGCAGGTCGACTCTAGAAAGCTTGGAGTTCTTCACCTCGGACTC | ||
a
Restriction sites are underlined.