Figure 2.
ANXA1 is overexpressed in thyroid cancer. (A) The protein expression level of ANXA1 in thyroid cancer tissues and matched normal tissues was detected using western blot analysis. **P<0.01 vs. N group. (B) The protein expression level of ANXA1 in the Nthy-ori3-1, TT and SW579 cell lines were detected using western blot analysis. **P<0.01 vs. Nthy-ori3-1. (C) The TT and SW579 cells were transfected with shRNA NC, ANXA1 shRNA1 and ANXA1 shRNA2, and western blot analysis was used to determine the protein expression level of ANXA1. **P<0.01 vs. shRNA NC. (D) The TT and SW579 cells were transfected with OE NC and ANXA1 OE plasmids, then western blot analysis was used to determine the protein expression level of ANXA1. **P<0.01 vs. OE NC. N, normal tissues; T, tumor tissues; sh, short hairpin; OE, overexpression; NC, negative control; ANXA1, annexin A1.