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. 2021 Nov 11;59(6):104. doi: 10.3892/ijo.2021.5284

Figure 5.

Figure 5

ANXA1 is transferred from the SW579 cells to the Nthy-ori3-1 cells via exosomes. Identification of the exosomes derived from the Nthy-ori3-1 cells and SW579 cells using (A) transition electron microscopy, (B) Nanoparticle Tracking Analysis and (C) western blot analysis. (D) The protein expression level of ANXA1 was analyzed using western blot analysis in exosomes isolated from the Nthy-ori3-1 and SW579 cells and the results were (E) statistically analyzed. **P<0.01 vs. Nthy-ori3-1-Exo. (F) SW579 cell-derived exosomes (Red) absorbed by the Nthy-ori3-1 cells were observed under a confocal fluorescence microscope. (G) The SW579 cells transfected with Cy3-tagged ANXA1 were co-cultured with the Nthy-ori3-1 cells for 48 h, then the Cy3-tagged ANXA1 absorbed by the Nthy-ori3-1 cells were observed under a fluorescence microscope. (H) The SW579 cells were transfected with ANXA1 shRNA2 or ANXA1 OE plasmids, then the conditioned medium was co-cultured with the Nthy-ori3-1 cells. The protein expression level of ANXA1 was subsequently analyzed. **P<0.01. sh, short hairpin; OE, overexpression; NC, negative control; ANXA1, annexin A1; Exo, exosomes.