Figure 7.

Exosomal ANXA1 promotes the malignant transformation of the Nthy ori3-1 cells via the activation of TGF-β/Smad2 and Akt signaling. The SW579 cells were transfected with ANXA1 shRNA2, ANXA1 OE or NC plasmids, then the Nthy-ori3-1 cells were co-cultured with exosomes isolated from the conditioned medium from the transfected SW579 cells. The protein expression level of (A) p-Akt and Bax, and (B) p-Smad2, E-cadherin and N-cadherin in the Nthy-ori3-1 cells were determined using western blot analysis. ^*P<0.05, ^**P<0.01. sh, short hairpin; OE, overexpression; NC, negative control; ANXA1, annexin A1; Exo, exosomes; p, phosphorylated.