Table 3.

Binding Affinity of HSA for PFAS^a

PFAS	CAS ID	chain length	aliphatic carbons	fluorines	R 2	ΔTm (°C)	EC50 (mM)	Kd (mM)
PFCA
PFBA	375-224	3	4	7	0.94	6.48 ± 0.18	2.61 ± 0.47	2.57 ± 0.78
PFPeA	2706-90-3	4	5	9	0.97	13.1 ± 0.066	2.14 ± 0.42	2.10 ± 0.56
PFHxA	307-244	5	6	11	0.98	10.6 ± 0.24	1.40 ± 0.27	1.64 ± 0.47
PFHpA	375-86-9	6	7	13	0.95	15.3 ± 0.41	0.68 ± 0.15	0.44 ± 0.20
PFOA	335-67-1	7	8	15	0.97	13.5 ± 0.41	0.84 ± 0.15	0.83 ± 0.38
PFNA	375-95-1	8	9	17	0.97	13.4 ± 0.34	0.60 ± 0.23	0.58 ± 0.21
PFDA	335-76-2	9	10	19	0.99	17.2 ± 0.13	1.11 ± 0.17	1.19 ± 0.59
PFunDA	2058-94-8	10	11	21	0.98	9.02 ± 0.47	1.49 ± 0.048	1.36 ± 1.06
PFDoA	307-55-1	11	12	23	0.98	7.74 ± 0.20	2.51 ± 0.34	1.89 ± 0.64
PFSA
PFBS	375-73-5	4	4	9	0.96	11.9 ± 0.11	1.72 ± 0.56	1.65 ± 0.69
PFHxS	3871-99-6	6	6	13	0.98	11.0 ± 0.24	0.98 ± 0.069	0.71 ± 0.47
PFOS	2795-39-3	8	8	17	0.98	16.2 ± 0.90	1.13 ± 0.32	0.69 ± 0.078
Per- and Polyfluorinated Alkyl Ethers
E1	3330-15-2	5	5	11	0.93	7.32 ± 0.059	2.34 ± 0.56	1.64 ± 0.34
HFPO-DA	13252-13-6	5	5	11	0.97	13.7 ± 0.17	1.83 ± 0.87	1.60 ± 0.55
Nafion bp2	749836-20-2	7	7	14	0.98	12.4 ± 0.17	1.90 ± 0.59	1.51 ± 0.37
PFO3DoDA	151772-59-7	6	7	13	0.95	20.6 ± 0.82	1.67 ± 0.47	1.53 ± 0.34
Fluorotelomer Alcohols
4:2 FTOH	204 3 47-2	4	6	9	N/A	0 ± 0	N/A	N/A
6:2 FTOH	64742-7	6	8	13	N/A	0 ± 0	N/A	N/A
Fluorotelomer Carboxylic Acids
3:3 FTCA	356-02-5	3	6	7	0.81	2.24 ± 0.062	2.06 ± 0.51	1.71 ± 0.47
5:3 FTCA	91463749-3	6	8	11	0.94	3.62 ± 0.14	1.48 ± 0.24	0.62 ± 0.10
6:3 FTCA	27854-304	6	9	13	0.95	9.50 ± 0.20	0.84 ± 0.21	0.81 ± 0.23
8:3 FTCA	83310-584	8	11	17	0.89	10.01 ± 0.17	1.16 ±0.42	0.97 ±0.17
Fluorotelomer Sulfonic Acids
4:2 FTSA	757124-724	4	6	9	0.93	3.49 ± 0.12	1.45 ± 0.34	1.07 ± 0.47
6:2 FTSA	59587-38-1	6	8	13	0.91	3.60 ± 0.47	0.47 ± 0.29	0.37 ± 0.34

^aChain length is the number of per/polyfluorinated carbons; ΔT_m is in °C, EC₅₀ and K_d are mean values reported in + SD. Each compound was run on at least two separate plates with n ≥ 4.