Table 3. Primers used in this study.
| Primer name | Sequence (5' to 3') | Purpose | Reference |
|---|---|---|---|
| ESBL15_repl_gibs_for | GCC AGT TAA TAG TTT GCG CAA CGT TGT TGC CAT TGC TGC ACT GAG CTA CCA TAG ATG AC |
PCR amplification of IncI1 replicon from pEBSL for Gibson assembly | This study |
| ESBL15_repl_gibs_rev | TAC AAT TAT TCC TTA CTA TGG ACA AAA ACA TCA ATC TGA T GTG GTT TCA GAA CGG TGA G |
PCR amplification of IncI1 replicon from pEBSL for Gibson assembly | This study |
| ESBL15_ori_ver_up | CCA GTT AAT AGT TTG CGC AAC |
Validation of pCP20-IncI1 cloning | This study |
| ESBL15_ori_intl_ rev | CTT TCA GCG CTT TAT AGC G |
Validation of pCP20-IncI1 cloning | This study |
| ESBL15_ori_intl_for | CTG TTC CGA TGA CCA TCT G |
Validation of pCP20-IncI1 cloning | This study |
| ESBL15_ori_ver_dw | CTC CAG TTT AAA TAC AAG ACG |
Validation of pCP20-IncI1 cloning | This study |
| ssaV-137F | GCAGCGTTCCA GGGTATTCC |
Verification of ΔssaV in the chromosome | Bakkeren et al., 2021a |
| ssaV +155 R | CAGCAAGTTCT TCTCCAGGC |
Verification of ΔssaV in the chromosome | Bakkeren et al., 2021a |
| invG-134F | GAAGGCCACGA GAACATCAC |
Verification of ΔinvG in the chromosome | Bakkeren et al., 2021a |
| invG +112 R | GCGGCCTGTT GTATTTCCGC |
Verification of ΔinvG in the chromosome | Bakkeren et al., 2021a |
| P3_SmR_for | CTA GTA TGA CGT CTG TCG C |
Verification of P3 | This study |
| P3_SmR_rev | CAC GTT TCG CAA CCT GTT C |
Verification of P3 | This study |
| WITS1 | ACG ACA CCA CTC CAC ACC TA |
qPCR for TAG1 | Grant et al., 2008 |
| WITS2 | ACC CGC AAT ACC AAC AAC TC |
qPCR for TAG2 | Grant et al., 2008 |
| WITS11 | ATC CCA CAC ACT CGA TCT CA |
qPCR for TAG11 | Grant et al., 2008 |
| WITS13 | GCT AAA GAC ACC CCT CAC TCA |
qPCR for TAG13 | Grant et al., 2008 |
| WITS17 | TCA CCA GCC CAC CCC CTC A |
qPCR for TAG17 | Grant et al., 2008 |
| WITS19 | GCA CTA TCC AGC CCC ATA AC |
qPCR for TAG19 | Grant et al., 2008 |
| WITS21 | ACA ACC ACC GAT CAC TCT CC |
qPCR for TAG21 | Grant et al., 2008 |
| ydgA | GGC TGT CCG CAA TGG GTC |
qPCR for all tags | Grant et al., 2008 |
| pagJ_fwd | ATC TTC GGG GAA GGG CAC GTC CG |
14028S chromosome-specific PCR | This study |
| pagJ_rev | GCT GTA ACC GTA AGG ATA GTG TGC CAC AAT T |
14028S chromosome-specific PCR | This study |
| cat_fwd | GCATTTCAGTCA GTTGCTCAATGT ACCTATAACC |
P2cat-specific PCR | This study |
| cat_rev | CGACATGGAAGC CATCACAAACGG |
P2cat-specific PCR | This study |