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. 2021 Nov 24;11:779739. doi: 10.3389/fonc.2021.779739

Figure 2.

Figure 2

dsRNA staining is significantly lower under hypoxia independently of HIF1α/2α expression. (A) dsRNA was stained using J2 antibody in MCF7 cells exposed to normoxia (n=45 cells) or 0.1% hypoxia (n=45 cells) for 48h from 3 independent replicates. (B) dsRNA was monitored during a time course of MCF7 cells in normoxia (n=40 cells), or exposed to 0.1% for 4h (n=40 cells), 16h (n=40 cells), and 48h (n=40 cells) from 3 independent replicates. (C) HIF1α/2α involvement was evaluated by staining dsRNA in 786-0 WT cells (786 WT, n=46 normoxic cells and n=46 hypoxic cells), and 786-0 HIF2α-KO cells (786 KO, n= 45 normoxic cells and n=45 hypoxic cells) from 3 independent replicates. Data is shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Green: J2 antibody staining, blue: DAPI, and red: MitoTracker staining. White arrow: dsRNA inside of mitochondria, blue arrow: dsRNA outside of mitochondria. Scale bars correspond to 10μm.