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. 2021 Nov 24;11:779739. doi: 10.3389/fonc.2021.779739

Figure 5.

Figure 5

Hypoxia downregulates the expression of mitochondrial genes. (A) RNA expression of mitochondrial encoded genes (12S, ND3, ATP6, CYTB) or nuclear encoded genes involved in mitochondrial function (POLRMT, TFAM, TFB1M) in MCF7 cells cultured in normoxia or 0.1% hypoxia for 4h, 8h, 16h, 24h and 48h was evaluated by qPCR (n=3). (B) Heatmap showing expression of mitochondrial encoded genes (right panel) or 1158 nuclear encoded genes involved in mitochondrial function (left panel, from MitoCarta 2.0) in MCF7 cells cultured in normoxia or 0.1% hypoxia for 48h (n=3) from RNAseq experiment described in Materials section. Number of replicates indicate biological replicates and data is shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.