Table 1.
No. | Binding sites (5′−3′)a | KD (μM)b |
dhdO 0# | GTTATCAGATAAC | 0.64 ± 0.10 |
dhdO 1# | TTTATCAGATAAC | 3.29 ± 0.72 |
dhdO 2# | GGTATCAGATAAC | 2.67 ± 0.53 |
dhdO 3# | GTGATCAGATAAC | 1.19 ± 0.25 |
dhdO 4# | GTTCTCAGATAAC | 0.58 ± 0.09 |
dhdO 5# | GTTAGCAGATAAC | 1.14 ± 0.37 |
dhdO 6# | GTTATAAGATAAC | 0.73 ± 0.18 |
dhdO 7# | TGTATCAGATAAC | 5.81 ± 2.15 |
dhdO 8# | TTGATCAGATAAC | 4.02 ± 0.92 |
dhdO 9# | TTTCTCAGATAAC | 3.23 ± 1.27 |
dhdO 10# | TTTAGCAGATAAC | n.d.c |
dhdO 11# | TTTATAAGATAAC | 3.23 ± 1.41 |
dhdO 12# | GGGATCAGATAAC | 6.25 ± 1.82 |
dhdO 13# | GGTCTCAGATAAC | 2.46 ± 0.51 |
aThe point mutation is shown in bold letter. The 1-bp interval in the palindrome sequence is shown in underlined letter.
bThe KD value was determined by ITC and analyzed by using a single-site binding model based on the raw data in Supplementary Fig. 13.
cn.d. not detected.