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. 2021 Dec 7;12:7108. doi: 10.1038/s41467-021-27357-7

Table 1.

Influence of point mutations in DBS on affinity between dhdO and DhdR.

No. Binding sites (5′−3′)a KD (μM)b
dhdO 0# GTTATCAGATAAC 0.64 ± 0.10
dhdO 1# TTTATCAGATAAC 3.29 ± 0.72
dhdO 2# GGTATCAGATAAC 2.67 ± 0.53
dhdO 3# GTGATCAGATAAC 1.19 ± 0.25
dhdO 4# GTTCTCAGATAAC 0.58 ± 0.09
dhdO 5# GTTAGCAGATAAC 1.14 ± 0.37
dhdO 6# GTTATAAGATAAC 0.73 ± 0.18
dhdO 7# TGTATCAGATAAC 5.81 ± 2.15
dhdO 8# TTGATCAGATAAC 4.02 ± 0.92
dhdO 9# TTTCTCAGATAAC 3.23 ± 1.27
dhdO 10# TTTAGCAGATAAC n.d.c
dhdO 11# TTTATAAGATAAC 3.23 ± 1.41
dhdO 12# GGGATCAGATAAC 6.25 ± 1.82
dhdO 13# GGTCTCAGATAAC 2.46 ± 0.51

aThe point mutation is shown in bold letter. The 1-bp interval in the palindrome sequence is shown in underlined letter.

bThe KD value was determined by ITC and analyzed by using a single-site binding model based on the raw data in Supplementary Fig. 13.

cn.d. not detected.