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. 2021 Dec 7;11:23549. doi: 10.1038/s41598-021-02729-7

Figure 3.

Figure 3

NMac1 treatment in the absence of glucose induces AMPK activation and inhibits mTOR and ERK. (a) Representative images of western blot analysis for AMPK/mTOR/ERK signaling changes and (b) quantified relative expressions using Multigauge ver.3.0 in MDA-MB-231 cells. Indicated concentrations of NMac1 were treated for 12 h under the presence and absence of glucose. Error bars represent SD (n = 3). *p < 0.05, **p < 0.01. (c) Kinetics of AMPK/mTOR/ERK signaling changes by western blot analysis. MDA-MB-231 cells were treated with 12.5 μM NMac1 under the indicated condition. Full-length blots are presented in Supplementary Fig. 7.