Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 7;9:50. doi: 10.1038/s41413-021-00173-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Runx1 knockout aggravates articular cartilage destruction in an ACLT-induced mouse OA model. a Schematic diagram showing the generation of Runx1-knockout mice and the detailed sample collection procedure. We achieved chondrocyte-specific Runx1 knockout by intraperitoneal tamoxifen injection at 3–5 days after recovery from ACLT surgery. We performed intraperitoneal tamoxifen injection twice per week. After 2 weeks of injections, cartilage genotyping was performed to determined whether Runx1 knockout was achieved. b Western blotting and quantification showing the expression of Runx1 in the total joint and articular cartilage of Runx1-knockout mice. c In vivo imaging showing the joint destruction in Runx1-knockout mice at 12 weeks after ACLT surgery. d H&E staining showing pathological changes in articular cartilage in Runx1-knockout mice at 12 weeks after ACLT surgery. e Masson staining showing pathological changes in articular cartilage in Runx1-knockout mice at 12 weeks after ACLT surgery. f IHC staining showing the expression of Col2a1 in articular cartilage in Runx1-knockout mice at 12 weeks after ACLT surgery. g Immunofluorescence showing the expression of SOX9 in articular cartilage in Runx1-knockout mice at 12 weeks after ACLT surgery. h Safranin O staining showing the loss of proteoglycan in articular cartilage in Runx1-knockout mice at 12 weeks after ACLT surgery. i The degree of experimental mouse OA was evaluated by scoring cartilage destruction (Osteoarthritis Research Society International (OARSI) grade), synovitis, osteophyte maturity, and subchondral bone plate (SBP) thickness (suggestive of sclerosis). Runx1^f/f/Δ Runx1 knockout. These results are based on at least three independent experiments (n = 3). The standard Mann–Whitney U test was used for the OARSI grade, synovitis, osteophyte maturity and SBP thickness, and the data in i (right) are shown as box (from 25%, 50% to 75%) and whisker (minimum to maximum values) plots. All significance data presented in b and i are based on two-tailed Student’s t-tests