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. 2021 Dec 7;12:7094. doi: 10.1038/s41467-021-27428-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a mRNA levels of FABP family members in RAW264.7 cells as determined by qPCR, n = 3 biologically independent samples over three independent experiments, data are presented as mean ± SEM and analyzed with a 95% confidence interval, P <0.0001, N.D, not detected, one-way ANOVA is followed by Tukey’s post-hoc test. b Protein levels of FABP5 in macrophages (MH-S), lung cancer cells (A549), epithelial cells (MLE-12), endothelial cells (HUVEC), fibroblasts (HFL1), and neutrophils (primary neutrophils) as evaluated by immunoblot. c Levels of FABP5 evaluated by immunoblot in RAW264.7 cells exposed to H₂O₂ (200 μM) for indicated time (β-actin, loading control). d mRNA levels of Fabp5 in RAW264.7 cells after exposure to H₂O₂ (200 μM) for indicated time as determined by qPCR, n = 3 biologically independent samples over three independent experiments, data are shown as mean ± SEM and analyzed with a 95% confidence interval, one-way ANOVA is followed by Tukey’s post-hoc test. e Co-IP of S-glutathionylation of FABP5 in COS-7 cells co-transfected with pXJ40-3xFlag-FABP5 and pcDNA3.1-3xFlag-Grx1 (or vector) and exposed for 15 min to H₂O₂ (200 μM) at 24 h post-transfection (IP, GSH; IB, FABP5). Whole cell lysates confirm the expression of FABP5, Grx1, and β-actin (DTT, negative control). f Co-IP showing S-glutathionylation of FABP5 in BMDMs (from control or Grx1 KO mice) after exposure to H₂O₂ (200 μM) for 15 min (IP, GSH; IB, FABP5). Whole cell lysates confirm the expression of FABP5, Grx1, and β-actin (DTT, negative control). g Immunoblotting analysis of reducing or non-reducing SDS–PAGE of RAW264.7 cells after exposure to H₂O₂ (at the indicated concentration) for 15 min. h COS-7 cell lysates subjected to SDS-PAGE under reducing or non-reducing conditions after transfection of pXJ40-3xFlag vector or pXJ40-3xFlag-FABP5 and exposure to H₂O₂ (200 μM) for 15 min. i Co-IP for S-glutathionylation of FABP5 in WT BMDMs after treatment with LPS (100 ng/mL) for 15 min (IP, GSH; IB, FABP5). Whole cell lysates confirm the expression of FABP5 and β-actin. j S-glutathionylation of FABP5 in lung tissues from Grx1^fl/fl and Grx1^fl/flLysM^cre mouse 24 h following intratracheal administration of PBS or LPS. Source data are provided as a Source Data file.