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. 2021 Dec 7;11:23566. doi: 10.1038/s41598-021-02936-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Elongation index and frequency of asymmetrical RBC in 10 and 60 Pa shear conditions. While the ensemble average elongation index remains unchanged for both conditions, the fraction of asymmetrical RBC in the 60 Pa increases beyond ~ 100 s of cumulative exposure, while the 10 Pa condition remains unchanged. Asymmetrical RBC are presented as a percentage of total cell counts per frame.