Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 8;7:379. doi: 10.1038/s41420-021-00715-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A Western blot assay of sh-OGT knockdown efficiency in A2780/DDP cells. A2780/DDP cells were treated with inhibitor-NC + sh-NC, miR-181d inhibitor + sh-NC, or miR-181d inhibitor + sh-OGT. B RT-qPCR detection of miR-181d expression in A2780/DDP cells normalized to U6. C Western blot assay of OGT expression in A2780/DDP cells normalized to β-actin. D CCK-8 assay of A2780/DDP cell viability. E Flow cytometric detection of A2780/DDP cell apoptosis. F Western blot assay of pro-caspase3 and cleaved caspase-3 expression and quantitative analysis in A2780/DDP cells normalized to β-actin. G Western blot assay of PCNA expression and quantitative analysis in A2780/DDP cells normalized to β-actin. A2780 cells were treated with mimic-NC + oe-NC, miR-181d mimic + oe-NC, or miR-181d mimic + oe-OGT. H RT-qPCR detection of miR-181d expression in A2780 cells normalized to U6. I Western blot assay of OGT expression in A2780 cells normalized to β-actin. J CCK-8 assay of A2780 cell viability. K Flow cytometric detection of A2780 cell apoptosis. L Western blot assay of pro-caspase3 and cleaved caspase-3 expression and quantitative analysis in A2780 cells normalized to β-actin. M Western blot assay of PCNA expression and quantitative analysis in A2780 cells normalized to β-actin. Cell experiments were repeated three times independently. *p < 0.05 versus A2780/DDP cells treated with sh-NC, or inhibitor-NC + sh-NC or A2780 cells treated with mimic-NC + oe-NC. #p < 0.05 versus A2780/DDP cells treated with miR-181d inhibitor + sh-NC or A2780 cells treated with miR-181d mimic + oe-NC.