Skip to main content
. 2021 Dec 8;7:379. doi: 10.1038/s41420-021-00715-6

Fig. 7. miR-181d regulates the OGT/KEAP1/NRF2 axis to promote OC Resistance to DDP in vivo.

Fig. 7

A2780 cells stably transfected with mimic-NC + sh-NC, miR-181d mimic + sh-NC or miR-181d mimic + sh-NRF2 and untreated A2780 cells were xenografted subcutaneously into the DDP-treated nude mice. A Tumor volume of nude mice measured at 5, 10, 15, 25 days after cell injection. B Representative images showing xenografts in nude mice. C Quantitative analysis of panel B. D RT-qPCR detection of miR-181d expression in tumor tissues of nude mice normalized to U6. E Western blot assay of the expression and quantitative analysis of NRF2, KEAP1, and OGT proteins in nude mouse tumor tissues normalized to β-actin. *p < 0.05 versus nude mice treated with PBS. #p < 0.05 versus nude mice treated with DDP + mimic-NC + sh-NC. & p < 0.05 versus nude mice treated with DDP + miR-181d mimic + sh-NC. n = 6 for nude mice in each group.