Fig. 3.

Comparative analysis of gene expression between head regeneration and budding in Hydra. (A) PCA plot of Hydra gene expression during head regeneration and budding shows that the developmental trajectories of head regeneration and budding converge along PC1. The dotted lines show the overall trajectories of regeneration and budding time courses. (B) Heatmap of the 298 DE genes, grouped in eight clusters based on similar expression dynamics during head regeneration and budding. (C) Median expression profiles of clusters in the time courses of regeneration (red profiles) and budding (green profiles). The clusters correspond to figure 3B and the number of genes in each cluster are shown inset. (D) Representative enriched GO terms for the clusters of DE genes in figure 3B. Significance level is at FDR of 0.05. Clusters 1, 3–4, and 7–8 did not have any enriched GO terms.