Figure 6.
Addition of oleate/palmitate rescues the kynurenine-induced T cell apoptosis phenotype. Murine Tconv were stimulated with CD3ε/CD28 mAb-coated beads ±1 mM L- or D-kynurenine for three days and proliferation assessed through CFSE-dilution. (a) Addition of oleate/palmitate rescued Tcell proliferation from kynurenine-induced T cell apoptosis. Data representative of three independent experiments. (b, c) Similar to the addition of oleate/palmitate, use of lipid-free bovine serum albumin (vehicle control for oleate/palmitate) and using 2 × 105 instead of 105 Tconv per well (200 μL) partially rescued the T cell proliferation (b) and apoptosis (c) phenotype induced by kynurenine exposure. Data representative of three independent experiments.