Figure 3.

The effects of intermittent fasting and melatonin supplementation on adipocytes morphology in mice. (A) Representative H&E staining of epididymal WAT sections. Scale bar: 50 μm; (B) quantification of adipocyte size; (C) quantification of adipocyte number per square millimeter. Data are presented as mean ± SEM, n = 6. Labeled means without a common letter differ (P < 0.05), NS: P ≥ 0.05. C, control; F, intermittent fasting; M, melatonin; MF, intermittent fasting plus melatonin. The C and M mice were fed ad libitum, while the F and MF groups underwent alternative-day feed deprivation. The M and MF groups mice were supplemented with melatonin at a dose of 10 mg/kg body weight by drinking.