Figure 9.

Significantly altered intestinal metabolites between in control mice and those treated with intermittent fasting and melatonin. (A) 4-hydroxybutyrate abundance. (B) 3-hydroxybutyric acid abundance. Data are presented as mean ± SEM, n = 12; Labeled means without a common letter differ (P < 0.05), NS: P ≥ 0.05. C, control; F, intermittent fasting; M, melatonin; MF, intermittent fasting plus melatonin. The C and M mice were fed ad libitum, while the F and MF groups underwent alternative-day feed deprivation. The M and MF groups mice were supplemented with melatonin at a dose of 10 mg/kg body weight by drinking.