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. 2021 Nov 24;9:719993. doi: 10.3389/fcell.2021.719993

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Copyright © 2021 Chen, Cheng, Zou, Wang, Wang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ADAMTS9-AS1 restrains cancer cell invasion and proliferation. ADAMTS9-AS1 was overexpressed in MDA-MB-231 and BT-549, while being silenced in MCF7. (A) Level of ADAMTS9-AS1 in breast cancer cells. (B) Results of in vitro colony formation method. (C) Results of EdU method (400×). (D) Cell invasion results (100×). (E) The expression of Ki67, PCNA, MMP-2 and MMP-9 after interfering with ADAMTS9-AS1 expression. (F) ADAMTS9-AS1 level in cancer cells. (G) Proliferation of cancer cells. (H) Results of EdU method (400×). (I) Transwell method is used to test cell invasion (100×). (J) The expression of Ki67, PCNA, MMP-2 and MMP-9. *p < 0.05.