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. 2021 Nov 24;12:729209. doi: 10.3389/fimmu.2021.729209

Figure 4.

Figure 4

Long-term priming induces lactate resistance. (A) Intracellular and (B) supernatant L-lactate concentration after a six- or 24-hour priming with 10mM Na-L-lactate. (C) Basal glycolytic efflux, (D) glycolytic reserve, (E) basal respiration, and (F) activity of complex I of the respiratory chain after a 24-hour priming with 10mM Na-L-lactate. (G) IL-6 secretion and (H) intracellular L-lactate concentration after 24 hours priming with Na-L-lactate as indicated and subsequently stimulation for six hours with LPS in the presence of fresh 10mM Na-L-lactate as indicated. Data are shown as (A, E, F) mean + SD or (B, D, G, I) mean + SEM (n=5; except for (B) n=4 and (F) n=3). Group comparisons were performed by (C, D) unpaired t test or (G, H) one-way ANOVA followed by Fisher’s LSD test. (A, B, E, F) One sample t tests were performed against the theoretical values 100 or 10 respectively (***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05).