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. 2021 Nov 24;12:770787. doi: 10.3389/fmicb.2021.770787

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Copyright © 2021 Sutela, Piri and Vainio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genome organization and phylogenetic analysis of Heterobasidion ambi-like viruses 1–4 (HetAlV1–4). (A) Schematic presentation of HetAlV1–4 genomes with UTRs and predicted ORFs with translation initiation and termination sites. Green-colored ORFs represent the putative RdRPs. (B) Phylogenetic tree of the ambi-virus like viruses. The predicted aa sequences of putative RdRPs were aligned in MEGA X (Kumar et al., 2018) with MUSCLE and tree was constructed with the LG (+F) model with G+I rates among sites (best-fit model according to ModelFinder) and 500 bootstrap repetitions. All positions with less than 95% site coverage were eliminated, i.e., fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. The Heterobasidion ambi-like viruses are designated with a star.