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. 2021 Nov 24;15:786076. doi: 10.3389/fnins.2021.786076

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Copyright © 2021 Anoar, Woodling and Niccoli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GAL4/UAS bipartite system of expression. The driver line contains a tissue specific promoter (in this case the neuronal specific elav promoter), driving the expression of yeast GAL4 protein in neurons. The responder line carries the gene of interest cloned down-stream of the UAS promoter, this, however, is not expressed. When the driver line is crossed to the responder line, the GAL4 driver and the UAS-transgene construct will come together in one animal. The GAL4 will be expressed in neurons, bind the UAS promoter and drive the expression of the gene of interest specifically in neurons. Images created with BioRender.com.