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Specificity of PCR amplification. Specific amplification of the 721-bp DNA fragment was detected only in B. thetaiotaomicron isolates. Lane, M, 100-bp DNA ladder (Gibco BRL); lanes 1 to 6, 8, 9, 12, and 14, B. thetaiotaomicron isolates; lane 7, B. fragilis; lane 10, B. distasonis; lane 11, B. uniformis; lane 13, B. vulgatus.
