Dot blot hybridization showing the specificity of PCR amplification. All positive hybridization reactions (indicated by the presence of a hybridization signal) were obtained with B. thetaiotaomicron isolates. All negative reactions (no hybridization signal) were from other species as follows: B. ovatus (1D), B. uniformis (2D), B. distasonis (3B), B. fragilis (3D), B. vulgatus (5B), Staphylococcus aureus (5E), Streptococcus bovis (6A), Enterococcus faecalis (6C), and Escherichia coli (6E).