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. 2021 Nov 24;12:750142. doi: 10.3389/fgene.2021.750142

TABLE 1.

qPCR primer sequences for the gene expression assay.

Target genes Primer sequence Accession number
Dnmt1 Forward: AAC​GGA​ACA​CTC​TCT​CTC​ACT​CA NM_053354
Reverse: TCA​CTG​TCC​GAC​TTG​CTC​CTC
Dnmt3a Forward: CAG​CGT​CAC​ACA​GAA​GCA​TAT​CC NM_001003958
Reverse: GGT​CCT​CAC​TTT​GCT​GAA​CTT​GG
Dnmt3b Forward: GAA​TTT​GAG​CAG​CCC​AGG​TTG NM_001003959
Reverse: TGA​AGA​AGA​GCC​TTC​CTG​TGC​C
Tet1 Forward: TGT​CAC​CTG​TTG​CAT​GGA​TT NM_001107643
Reverse: TTG​GAT​CTT​GGC​TTT​CAT​CC
Tet2 Forward: GAG​GAG​CAG​AAG​GAA​GCA​AG XM_227694.9
Reverse: CAC​CGT​AGC​AGA​ACA​GGA​AC
Tet3 Forward: CAG​GGA​CCA​AGC​AAC​AGA​AC XM_032906253.1
Reverse: AGG​GTG​TGA​GAG​GAA​AGA​GG
Gapdh Forward: TGC​ACC​ACC​AAC​TGC​TTA​GC NM_017008
Reverse: GGC​ATG​GAC​TGT​GGT​CAT​GAG
Kor Forward: AGC​TCT​TGG​TTC​CCC​AAC​TG NM_017167
Reverse: CAC​CAC​AGA​GTA​GAC​AGC​GG
Pdyn Forward: CCT​GTC​CTT​GTG​TTC​CCT​GT NM_019374
Reverse: AGA​GGC​AGT​CAG​GGT​GAG​AA