Table 1.
Formulation and proximate composition of the experimental diets (% dry matter).
| Ingredients (%) | Diets | ||||
|---|---|---|---|---|---|
| M0 | M2 | M4 | M8 | M16 | |
| Fish meal | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 |
| Shrimp meal | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Beer yeast powder | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Soybean meal | 30.00 | 30.00 | 30.00 | 30.00 | 30.00 |
| Dephenolized cottonseed protein | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Peanut meal | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 |
| Wheat flour | 22.00 | 22.00 | 22.00 | 22.00 | 22.00 |
| Fish oil | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Soybean oil | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Lecithin oil | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Monocalcium phosphate | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Choline chloride | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| Vitamin premix 1 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| Mineral premix 2 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Lysine hydrochloride | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 |
| Solid methionine | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 |
| Threonine | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| Vitamin C | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| MOS | 0 | 0.02 | 0.04 | 0.08 | 0.16 |
| Rice bran meal | 2.15 | 2.13 | 2.11 | 2.07 | 1.99 |
| Analyzed nutrients compositions (% dry matter) | |||||
| Crude protein | 43.64 | 43.28 | 44.41 | 44.07 | 43.00 |
| Crude lipid | 5.75 | 5.92 | 5.73 | 5.77 | 6.07 |
| Crude ash | 9.06 | 9.10 | 8.73 | 9.07 | 9.01 |
MOS, mannan oligosaccharide.
1Per kg vitamin premix contains: vitamin A acetate, 714,000 IU; vitamin D3, 266,000 IU; dl-α-tocopherol acetate, 8.6 g; menadione, 1 g; thiamine mononitrate, 1 g; riboflavin, 1.4 g; pyridoxine hydrochloride, 1.2 g; cyanocobalamin, 0.004 g; calcium d-pantothenate, 4 g; nicotinamide, 6.8 g; folic acid, 0.28 g; d-biotin, 0.012 g; inositol, 7.6 g; l-ascorbic acid-2-phosphate, 16.6 g.
2Per kg mineral premix contains: Mg, 12.5 g; Zn, 7.5 g; Mn, 2 g; Cu, 1.25 g; Co, 0.05 g; Se, 0.015 g; I, 0.05 g.