Figure 5.

Dynamics of peripheral PD-1 and PD-L1-expressing IgM⁺ memory B cells in R and NonR patients receiving anti-PD-1monotherapy. (A) Gating strategy for PD-1⁺ and PD-L1⁺ cells in IgM⁺ memory B cells by flow cytometry. (B) Comparisons of PD-1 expressions on IgM⁺ memory B cells, IgM^- memory B cells and IgM⁺ mature cells. (C) Comparisons of PD-L1 expressions on IgM⁺ memory B cells, IgM^- memory B cells and IgM⁺ mature cells. (D, E) Dynamics of the percentages of PD-1⁺IgM⁺ memory B cells (D) and PD-L1⁺IgM⁺ memory B cells (E) at the baseline and 8 weeks after nivolumab treatment in R (n = 14) and NonR (n = 18) patients. The paired-Student t test was used to analyze the differences between two groups as well as in baseline and after the treatment. ***P < 0.001, *P < 0.05, ns, P > 0.05.