Table 1.
Primers used in this study.
| Gene | Sequence (5′→3′) |
|---|---|
| Mice-GAPDH | Forward: GGAGAAACCTGCCAAGTATG Reverse: TGGGAGTTGCTGTTGAAGTC |
| Mice-IL-6 | Forward: CCAGTTGCCTTCTTGGGACT Reverse: GGTCTGTTGGGAGTGGTATCC |
| Mice-iNOS | Forward: GAGACAGGGAAGTCTGAAGCAC Reverse: CCAGCAGTAGTTGCTCCTCTTC |
| Promoter-PDC5 | Forward: CCCGCGGCCGCCAACAAAGGCCAAGGAAATAAAGCA Reverse: CATGAGTTTTATGTTAATTAGCTTATTTGTTCTTCT TGTTATTGTATTGTGTTGTTC |
| Terminator-PDC5 | Forward: GAACAACACAATACAATAACAAGAAGAACAAATAA GCTAATTAACATAAAACTCAT Reverse: CTTCAATTTAATTATATCAGTTATTACCCTGTAGTATTC GAGCATTAAGGA |
| Ura3-PDC5 | Forward: TCCTTAATGCTCGAATACTACAGGGTAATAACTGATAT AATTAAATTGAAG Reverse: TACCTAAGGTTATTTCAGACATGATTCGGTAATCTCCG AACAG |
| Part of the ORF-PDC5 | Forward: CTGTTCGGAGATTACCGAATCATGTCTGAAATAACCT TAGGTA Reverse: GGGCGGCCGCCGTTAGCAATATCAGTGATCA |
| Promoter-PDC6 | Forward: CCCGCGGCCGCGAGTTCACACCTTATTCACA Reverse: GTTTGAGTACACTACTAATGGCTTATTTGTTGGCA ATATGTTTTTGCTATATTACGTG |
| Terminator-PDC6 | Forward: CACGTAATATAGCAAAAACATATTGCCAACAAATAAGCC ATTAGTAGTGTACTCAAAC Reverse: CTTCAATTTAATTATATCAGTTATTACCCATGCAGAATGA GCACTTGTTA |
| Ura3-PDC6 | Forward: TAACAAGTGCTCATTCTGCATGGGTAATAACTGATAT AATTAAATTGAAG Reverse: TCCAAGAGTAATTTCAGACATGATTCGGTAATCTCCG AACAGAAGGAA |
| Part of the ORF-PDC6 | Forward: CTTCTGTTCGGAGATTACCGAATCATGTCTGAAATTAC TCTTGGA Reverse: GGGGCGGCCGCGTTGTCCTGATCAACCTATC |
| Promoter-ADH4 | Forward: CCCGCGGCCGCGCCTTCAATGAGCTTCCAT Reverse: TTGACGTTTATGAGTTCGTTCGATTTTTTTATTTTCTTAT TTGACTATTAGTTGTTGCA |
| Terminator-ADH4 | Forward: TGCAACAACTAATAGTCAAATAAGAAAATAAAAAAATC GAACGAACTCATAAACGTCAA Reverse: CTTCAATTTAATTATATCAGTTATTACCCAAGGAAGACA ATATCATTGACAG |
| Ura3-ADH4 | Forward: TGTCAATGATATTGTCTTCCTTGGGTAATAACTGATATA ATTAAATTGAAG Reverse: AAACCCAGTAACGGAAGACATGATTCGGTAATCTCCG AACAGAAGGAA |
| Part of the ORF-ADH4 | Forward: TTCTGTTCGGAGATTACCGAATCATGTCTTCCGTTACTG GGTTT Reverse: GGGGCGGCCGCTGGCAAACCAAACATGGTAGA |
| Promoter-PDC1 | Forward: CCCGCGGCCGCCTGACTTTTCGTGTGATGAGG Reverse: GATCAATTGGTCCTTCAAAGTAGCCATTTTGATTG ATTTGACTGTGT |
| Terminator-PDC1 | Forward: TCCAAAAGGAATTGCAATTCTAAGCGATTTAATCTC TAATTATTAGTTAAAGT Reverse: CTTCAATTTAATTATATCAGTTATTACCCACAACTAGCTTGT CTTGAGCA |
| Ura3-PDC1 | Forward: TGCTCAAGACAAGCTAGTTGTGGGTAATAACTGATATAA TTAAATTGAAG Reverse: TTACCCAAAGTAATTTCAGACATGATTCGGTAATCTCCGA ACAGA |
| Part of the ORF-PDC1 | Forward: TCTGTTCGGAGATTACCGAATCATGTCTGAAATTACTTTGG GTAA Reverse: GGGGCGGCCGCTAAGTGGTTCTGATACATCTGTCA |
| LDH-ΔPDC1∷LDH | Forward-ACACAGTCAAATCAATCAAAATGGCTACTTTGAAGGA CCAATTGATC Reverse: ACTTTAACTAATAATTAGAGATTAAATCGCTTAGAATTG CAATTCCTTTTGGA |
| Promoter-ADH1 | Forward: CCCGCGGCCGCTCCATTTGCCATCTATTGAAGT Reverse: TTGGTCCTTCAAAGTAGCCATTGTATATGAGATAGTT GATTGT |
| Terminator-ADH1 | Forward: TCCAAAAGGAATTGCAATTCTAAGTTGACACTTCTA AATAAGCGAA Reverse: CTTCAATTTAATTATATCAGTTATTACCCAACGTCTTGAAG CTCAGGTAAG |
| Ura3-ADH1 | Forward: CTTACCTGAGCTTCAAGACGTTGGGTAATAACTGATATAAT TAAATTGAAG Reverse: ACACCTTTTTGAGTTTCTGGGATAGACATGATTCGGTAAT CTCCGAACAGA |
| Part of the ORF-ADH1 | Forward: TCTGTTCGGAGATTACCGAATCATGTCTATCCCAGAAACT CAAAAAGGTGT Reverse: GGGGCGGCCGCGTGATACCAGCACACAAGATG |
| LDH-ΔADH1∷LDH | Forward: ACAATCAACTATCTCATATACAATGGCTACTTTGAAGG ACCAA Reverse: TTCGCTTATTTAGAAGTGTCAACTTAGAATTGCAATT CCTTTTGGA |