TABLE 1.
Yeast strains
| Strain | Genotype | Reference or source |
|---|---|---|
| IH1792 | MATα lys1 | Herskowitz laboratory |
| IH1793 | MATa lys1 cry1 | Herskowitz laboratory |
| Sc252 | MATa ura3-52 leu2-3,112 ade1 | 35 |
| SY1817a | MATα ste3Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | 7 |
| SY2150a | MATα GAL1-STE3 mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | 7 |
| SY2555a | MATa GAL1-STE3 mfa1Δ mfa2Δ HIS33::FUS1-HIS3 | This work |
| SY2560a | MATα GAL1-STE3 rad16::GAL1-STE4 mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| SY2561a | MATa GAL1-STE3 rad16::GAL1-STE4 mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1050a | MATa asg7Δ::G418RGAL1-STE3 rad16::GAL1-STE4 mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1077a | MATa ste3Δ::LEU2 rad16::GAL1-STE4 mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1078a | MATa asg7Δ::G418Rste3Δ::LEU2 rad16::GAL1-STE4 mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1086a | MATa GAL1-STE3 asg7Δ::G418Rmfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1123a | MATa GAL1-STE3 lys2::FUS1-LacZ bar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1124a | MATa GAL1-STE3 asg7Δ::G418Rlys2::FUS1-LacZ bar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1125a | MATa GAL1-STE3Δ365 asg7Δ::G418Rbar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1131a | MATa GAL1-STE3 ASG7::ADH1-ASG7 lys2::FUS1-LacZ bar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1132a | MATα GAL1-STE3 | This work |
| NDY1140a | MATa GAL1-STE3 | This work |
| NDY1141a | MATa GAL1-STE3Δ365 ASG7::ADH1-ASG7 bar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1172a | MATa ste3Δ::LEU2 lys2::FUS1-LacZ bar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1173a | MATa ste3Δ::LEU2 asg7Δ::G418Rlys2::FUS1-LacZ bar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| NDY1174a | MATa ste3Δ::LEU2 ASG7::ADH1-ASG7 lys2::FUS1-LacZ bar1Δ mfa1Δ mfa2Δ HIS3::FUS1-HIS3 | This work |
| W303-1A | MATa ura3-1 leu2-3 his3-11,-15 trp1-1 ade2-1 can1-100 | Rothstein laboratory |
| W303-1Bb | MATα | Rothstein laboratory |
| NDY1089b | MATa asg7Δ::G418R | This work |
| NDY1171b | MATa ASG7::ADH1-ASG7 | This work |
| NDY1179b | MATα ASG7::ADH1-ASG7 | This work |
| NDY1200b | MATα GAL1-STE3Δ365 | This work |
| NDY1204b | MATα GAL1-STE3Δ365 ASG7::ADH1-ASG7 | This work |
| NDY1225b | MATα GAL1-STE3Δ365 ASG7::ADH1-ASG7 ste4Δ::LEU2 | This work |
| Y405b | MATa fus2Δ::URA3 | This work |
| Y411b | MATα fus2Δ::URA3 HIS3::FUS1-HIS3 mfa2Δ::FUS1-LacZ | This work |
| Y427b | MATa fus1Δ::URA3 HIS3::FUS1-HIS3 mfa2Δ::FUS1-LacZ | This work |
| Y428b | MATα fus1Δ::URA3 TRP1 | This work |
| Y2615b | MATa asg7Δ::G418Rfus2Δ::URA3 | This work |
| Y2617b | MATa asg7Δ::G418Rfus1Δ::URA3 | This work |
| NDY343 | MATα ste3Δ::LEU2 ura3 leu2 his4 bar1-1 | 28 |
| NDY349c | MATα GAL1-STE3Δ365 | This work |
| NDY1176 | MATa/α GAL1-STE3Δ365/STE3 ASG7+/ASG7+ | This work |
| NDY1178d | MATa/α GAL1-STE3Δ365/STE3 ASG7::ADH1-ASG7/ASG7 | This work |
| NDY1185d | MATα/α GAL1-STE3Δ365/STE3 ASG7+/ASG7+ | This work |
| NDY1186d | MATα/α GAL1-STE3Δ365/STE3 ASG7::ADH1-ASG7/ASG7 | This work |
a
Isogenic to Sc252.
b
Isogenic to W303-1A.
c
Isogenic to NDY343.
d
Isogenic to NDY1176.