TABLE 4.
Prezygote morphologies from fusion-defective matings
| Matinga | % Prezygotesb | % Abnormal prezygotesc |
|---|---|---|
| a fus1Δ × α fus1Δ | 9 ± 1 | 17 ± 2d |
| a fus1Δ asg7Δ × α fus1Δ | 10 ± 1 | 16 ± 5 |
| a fus2Δ × α fus2Δ | 12 ± 2 | 2 ± 1 |
| a fus2Δ asg7Δ × α fus2Δ | 9 ± 1 | 2 ± 1 |
Matings were for 2.5 h. Bilateral fus1 matings were between Y428 and either Y427 or Y2617. Bilateral fus2 matings were between Y411 and either Y405 or Y2615.
The percentages of total cells within the mating mixtures showing prezygotic morphology (dumbbells) were determined as for the percentages of zygotes for Table 3.
A total of 200 cells showing prezygotic morphology were identified and classified as being either morphologically normal or abnormal. Prezygotes were classified as abnormal if the two mating partners were seen to connect at an angle. The fractions of the 200 counted prezygotes found to be morphologically normal were determined for three experiments, and the means ± standard deviations are reported.
A somewhat higher percentage of the prezygotes from the bilateral fus1 matings were scored as being morphologically abnormal (relative to those from bilateral fus2 matings). These abnormal prezygotes appear to result from the fus1 partners connecting on an angle rather than via the typical tip-to-tip connection.