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. 2021 Nov 24;13:705594. doi: 10.3389/fnagi.2021.705594

Figure 9.

Figure 9

PAS staining of LPS in Corpora amylacea of AD and aging brains, and PAS staining of purified LPS. In control, PVWM LPS (A1) and PAS (A2) were co-localized (A3). IN AD PVWM there were more LPS stained vacuoles (B1) and PAS-stained Corpora amylacea (B2) which were mostly co-localized (B3). Most but not all LPS stained vesicles were PAS positive and therefore were Corpora amylacea (CA). PAS stained both E. coli J5 LPS and E. coli O111:B4 LPS at the higher concentrations (1,000 ng/μl and 500 ng/μl) with much greater staining intensity for E. coli J5 LPS than E. coli O111:B4 LPS at the same concentrations (C,D). When the LPS concentrations were 250 ng/μl, PAS only stained E. coli J5 LPS but not the E. coli O111:B4 LPS. However, the difference was not significant. When the LPS concentrations were 125 ng/μl, PAS did not stain either E. coli J5 LPS or E. coli O111:B4 LPS. Note: LPS, lipopolysaccharide; PAS, Periodic acid–Schiff, a marker for CA; J5, E. coli J5 LPS; B4, E. coli O111:B4 LPS; AD, Alzheimer's disease; Bar = 50 μm.