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. 2021 Nov 15;23(1):47. doi: 10.3892/etm.2021.10969

Figure 3.

Figure 3

AVA4746 causes the detachment of B-ALL cells from human VCAM-1. Primary B-ALL cell lines (A) LAX7R, (B) LAX53, (C) LAX56, (D) TXL3 and (E) ICN24, in addition to B-ALL cell lines (F) RS4;11, (G) SupB15, (H) KASUMI-2, (I) 697, (J) BEL-1, (K) BV173 and (L) RCH were seeded into 96-well plates coated with either human VCAM-1 (10 µg/ml) or 2% BSA as a control for 4 h prior to treatment with the DMSO control or AVA4746 (25 µM) overnight. The percentage (%) of viable adherent cells was calculated based on the cell counts using trypan blue exclusion which was then normalized to those in the VCAM-1-DMSO group. ****P<0.0001. Experiments were performed in triplicates. VCAM-1, vascular cell adhesion molecule-1; B-ALL, B-lineage acute lymphoblastic leukemia.