Figure 3.

Effects of SHO and GIN on inflammatory cell infiltration and airway inflammation in lung tissues. The right lung lobes were isolated at 24 h after the final OVA challenge. Next, 4-µm lung sections were stained with H&E to analyze inflammatory cell infiltration and inflammatory score. (A) Panels show H&E-stained lung sections obtained from the control (first), OVA (second), OVA + SHO (third) and OVA + GIN (fourth) groups. Magnification, x200; scale bar, 75 µm. (B) ASM thickness was evaluated with LAS AF Ink. (C) Total inflammatory score expressed as an average. Red arrow indicates measured thickness site of epithelial cells. Values are presented as mean ± SD (n=6). ^##P<0.01, ^###P<0.001 vs. control group; ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. OVA group. OVA, ovalbumin; SHO, 6-shogaol; GIN, 6-gingerol.