Skip to main content
. 2021 Nov 12;478(21):3957–3976. doi: 10.1042/BCJ20210585

Figure 3. Expression and function of SLC38A5 in TNBC cell lines derived from patient-derived xenografts.

Figure 3.

(A) RT-PCR analysis of SLC38A5 and SLC6A14 mRNA expression in three cell lines. (B) Transport activity of SLC38A5 in the TNBC cell line TXBR-100 as monitored by the uptake of serine, glycine, and glutamine as the substrates for the transporter. The transport function of SLC38A5 was monitored in an uptake buffer (pH 8.5) containing 5 mM tryptophan to suppress the involvement of SLC7A5 in the uptake and by comparing the uptake in the presence and absence of Li+. The Li+-stimulatable uptake under these uptake conditions was taken as the transport activity specific for SLC38A5. ***, P < 0.001 compared with uptake in the absence of Li+.