Figure 5.

Add‐in‐time MSR vaccine induces persistent humoral response with neutralization potential. Six to 8 weeks old BALB/c mice were immunized on day 0 and boosted on day 28 with MSRs loaded with 1 µg GM‐CSF, 25 µg MPLA with 1 µg N protein, 1 µg S1 protein, and 1 µg S2 protein added minute injections (N/S1/S2 MSR vaccine, n = 10) or a Sham vaccine containing GM‐CSF and MPLA only (n = 10). Serum IgG concentration against the S2 domain (a), S1 domain (b), and RBD (c) were measured over time by ELISA. P‐values represent the results of Friedman test with post hoc Wilcoxon for repeated measures comparing all timepoints to day 0 concentration. d) Neutralization titer – line represents data mean (n = 10). P‐values represent the results of Friedman test with post hoc Wilcoxon for repeated measures comparing all timepoints to day 14 titer.