Figure 6.
Single‐shot MSR vaccine formulations induce anti‐SARS‐CoV‐2 activity. Six to 8 weeks old BALB/c mice were single immunized with vaccine formulations shown in (a) on day 0 (n = 10). Total S1‐ (b), S2‐ (c), and RBD‐specific (d) serum IgG was measured using ELISA. P‐values (black) represent the results of Wilcoxon signed‐rank test between day 0 and day 233 for each group (in all 4 groups, P < 0.01). In (b–d), all groups were compared against each other at the final timepoint using Kruskal–Wallis test with P < 0.05 for (b), P < 0.01 for (c), and P < 0.0001 for (d), the P values of the post hoc analysis of the comparisons at day 233 are indicated in orange. The neutralization potential of immunized sera was assessed against SARS‐CoV‐2 pseudovirus. e) Kruskal–Wallis test P‐values were P < 1 × 10−7, 10−4, 10−4, and 10−5 for days 28, 56, 104, and 135, respectively. Post hoc Conover test P‐values for the comparison of each group with each other are depicted in the plot.