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Effect of ASG7 on signaling in STE3DAF cells treated with a-factor. The following strains were treated with a-factor (300 nM) for the indicated periods of time: H67-6C.Ba, a MATa STE3DAF ASG7 strain (lanes 1 to 4), and H67-6C.a7, a MATa STE3DAF asg7::URA3 strain (lanes 5 to 8). RNA was isolated, transferred to nitrocellulose, hybridized with a FUS1 probe, and rehybridized with a PGK1 probe.
