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Effect of ASG7 on localization of GFP-Ste4p in STE3DAF cells. The following strains were treated with α-factor (0.1 μM) for 2 h: AC17-2B, a MATa STE3DAF ste4::HIS3 ASG7 strain (ASG7), and AC17-2B.aL, a MATa STE3DAF ste4::HIS3 asg7::LEU2 strain (asg7Δ). Both strains contained the low-copy-number STE4-GFP plasmid pBTL49. The live cells were viewed by fluorescence microscopy using an FITC filter set.
