TABLE 1.
Yeast strains used in this study
| Strain | Genotypea | Reference or source |
|---|---|---|
| W3031A | MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 | R. Rothstein |
| W3031B | MATα | R. Rothstein |
| W3031A.Ba | MATa sst1::hisG | This study |
| H67-9D.Ba | MATa mfa1-Δ3::HIS3 mfa2-Δ2::HIS3 sst1::hisG | 3 |
| H67-9D.a7 | MATa mfa1-Δ3::HIS3 mfa2-Δ2::HIS3 sst1::hisG asg7::URA3 | This study |
| H67-6C.Ba | MATa mfa1-Δ3::HIS3 mfa2-Δ2::HIS3 STE3DAF2.5 sst1::hisG | 3 |
| H67-6C.a7 | MATa mfa1-Δ3::HIS3 mfa2-Δ2::HIS3 STE3DAF2.5 sst1::hisG asg7::URA3 | This study |
| AC17-7B | MATa mfa1-Δ3::HIS3 mfa2-Δ2::HIS3 sst1::hisG ste4::HIS3 | 17 |
| AC17-2B | MATa mfa1-Δ3::HIS3 mfa2-Δ2::HIS3 STE3DAF2.5 sst1::hisG ste4::HIS3 | 17 |
| AC17-2B.aL | MATa mfa1-Δ3::HIS3 mfa2-Δ2::HIS3 STE3DAF2.5 sst1::hisG ste4::HIS3 asg7::LEU2 | This study |
| H125-7D | MATa gpa1::TRP1 STE3DAF2.5 | This study |
| H125-7D.aH | MATa gpa1::TRP1 STE3DAF2.5 asg7::HIS3 | This study |
a
All strains other than W3031A are isogenic to W3031A.