FIG. 1.

Sequence elements identified adjacent to pM151 sequences after analysis of rescued plasmids. Indicated are the areas sequenced by using primer P1 (on the left side of the plasmid) and primer P2 (on the right side). For better comparison, different sequence element types (unique, rDNA, LTR, tRNA pbs, and Ty ORF) are differentiated by shading types (see the key); elements were not drawn to scale. Numbers below the elements refer to SGD genomic coordinates, while coordinates given above the Ty elements refer to those Ty1 element sequences which gave the best fit in BLASTN analysis (see Table 1). The numbers of nucleotides missing from the 5′ protruding single-stranded plasmid ends are given just above the plasmid junctions. Asterisks indicate samples obtained after irradiation with 50 Gy. (A) Integration event probably mediated by topoisomerase I. (B) Three plasmid integrations without cointegrating Ty sequences. These events were most likely mediated by Ty integrase, since they created a 5-bp target site duplication. (C) Apparent cointegration events of pM151 and Ty sequences. For MK26 the junction between chromosomal DNA and cointegrating Ty sequences is also shown. RDN, rDNA.