Figure 3.

X-band (9.42 GHz) frozen solution (77 K) EPR spectra showing the isolation of the spectrum corresponding to GSH-Cu(II)GHK by weighted subtraction of the spectrum of Cu(II)GHK (Figure S16) and GHK–Cu(II)GHK (Figure S17) from the spectrum obtained for 0.50 mM GHK with 0.45 mM Cu(II) in the presence of 1.0 mM GSH at 4 °C. Dashed vertical lines indicate the approximate position of the prominent Cu(II) hyperfine features of each coordination mode as a guide to the eye.