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Differential absorption spectra calculated for 0.50 mM GHK with 0.45 mM Cu(II) in the presence of 2.0 mM 2-mercaptoethanol, T = 10 °C (black line), 1.0 mM NaSCH3, T = 15 °C (red line), and 1.0 mM GSH, T = 5 °C (blue line) expressed as a difference between the first spectrum recorded after thiol introduction and Cu(II)GHK spectrum.
