FIG. 4.

Mlx and Mondo shuttle between the cytoplasmic and nuclear compartments. Expression vectors encoding FLAG-tagged Mlx and MondoA were transfected into NIH 3T3 cells. Their subcellular localization was determined by indirect immunofluorescence using anti-FLAG and anti-MondoA antibodies. (A and B) Two representative cells coexpressing Mlx and Mondo, respectively. (C) The subcellular distribution of Mlx and MondoA was quantified in the absence or presence (+) of leptomycin B. Nuc, nuclear; Cyto, cytoplasmic. The error bars indicate standard deviations.